Genome-wide analysis of protein-protein interactions and involvement of viral proteins in SARS-CoV replication.
Identifieur interne : 003106 ( Main/Exploration ); précédent : 003105; suivant : 003107Genome-wide analysis of protein-protein interactions and involvement of viral proteins in SARS-CoV replication.
Auteurs : Ji'An Pan [République populaire de Chine] ; Xiaoxue Peng ; Yajing Gao ; Zhilin Li ; Xiaolu Lu ; Yingzhao Chen ; Musarat Ishaq ; Dan Liu ; Marta L. Dediego ; Luis Enjuanes ; Deyin GuoSource :
- PloS one [ 1932-6203 ] ; 2008.
Descripteurs français
- KwdFr :
- MESH :
- génétique : Protéines virales.
- métabolisme : Protéines virales.
- physiologie : Protéines virales, Réplication virale, Virus du SRAS.
- Gènes rapporteurs, Génome viral, Liaison aux protéines, Techniques de double hybride, Transcription génétique.
English descriptors
- KwdEn :
- MESH :
- chemical , genetics : Viral Proteins.
- chemical , metabolism : Viral Proteins.
- physiology : SARS Virus, Viral Proteins, Virus Replication.
- Genes, Reporter, Genome, Viral, Protein Binding, Transcription, Genetic, Two-Hybrid System Techniques.
Abstract
Analyses of viral protein-protein interactions are an important step to understand viral protein functions and their underlying molecular mechanisms. In this study, we adopted a mammalian two-hybrid system to screen the genome-wide intraviral protein-protein interactions of SARS coronavirus (SARS-CoV) and therefrom revealed a number of novel interactions which could be partly confirmed by in vitro biochemical assays. Three pairs of the interactions identified were detected in both directions: non-structural protein (nsp) 10 and nsp14, nsp10 and nsp16, and nsp7 and nsp8. The interactions between the multifunctional nsp10 and nsp14 or nsp16, which are the unique proteins found in the members of Nidovirales with large RNA genomes including coronaviruses and toroviruses, may have important implication for the mechanisms of replication/transcription complex assembly and functions of these viruses. Using a SARS-CoV replicon expressing a luciferase reporter under the control of a transcription regulating sequence, it has been shown that several viral proteins (N, X and SUD domains of nsp3, and nsp12) provided in trans stimulated the replicon reporter activity, indicating that these proteins may regulate coronavirus replication and transcription. Collectively, our findings provide a basis and platform for further characterization of the functions and mechanisms of coronavirus proteins.
DOI: 10.1371/journal.pone.0003299
PubMed: 18827877
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream PubMed, to step Corpus: 001A56
- to stream PubMed, to step Curation: 001A56
- to stream PubMed, to step Checkpoint: 001B26
- to stream Ncbi, to step Merge: 001D61
- to stream Ncbi, to step Curation: 001D61
- to stream Ncbi, to step Checkpoint: 001D61
- to stream Main, to step Merge: 003193
- to stream Main, to step Curation: 003106
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Genome-wide analysis of protein-protein interactions and involvement of viral proteins in SARS-CoV replication.</title><author><name sortKey="Pan, Ji An" sort="Pan, Ji An" uniqKey="Pan J" first="Ji'An" last="Pan">Ji'An Pan</name><affiliation wicri:level="4"><nlm:affiliation>State Key Laboratory of Virology and Modern Virology Research Centre, College of Life Sciences, Wuhan University, Wuhan, People's Republic of China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>State Key Laboratory of Virology and Modern Virology Research Centre, College of Life Sciences, Wuhan University, Wuhan</wicri:regionArea><placeName><settlement type="city">Wuhan</settlement><region type="région">Hubei</region></placeName><orgName type="university">Université de Wuhan</orgName></affiliation></author><author><name sortKey="Peng, Xiaoxue" sort="Peng, Xiaoxue" uniqKey="Peng X" first="Xiaoxue" last="Peng">Xiaoxue Peng</name></author><author><name sortKey="Gao, Yajing" sort="Gao, Yajing" uniqKey="Gao Y" first="Yajing" last="Gao">Yajing Gao</name></author><author><name sortKey="Li, Zhilin" sort="Li, Zhilin" uniqKey="Li Z" first="Zhilin" last="Li">Zhilin Li</name></author><author><name sortKey="Lu, Xiaolu" sort="Lu, Xiaolu" uniqKey="Lu X" first="Xiaolu" last="Lu">Xiaolu Lu</name></author><author><name sortKey="Chen, Yingzhao" sort="Chen, Yingzhao" uniqKey="Chen Y" first="Yingzhao" last="Chen">Yingzhao Chen</name></author><author><name sortKey="Ishaq, Musarat" sort="Ishaq, Musarat" uniqKey="Ishaq M" first="Musarat" last="Ishaq">Musarat Ishaq</name></author><author><name sortKey="Liu, Dan" sort="Liu, Dan" uniqKey="Liu D" first="Dan" last="Liu">Dan Liu</name></author><author><name sortKey="Dediego, Marta L" sort="Dediego, Marta L" uniqKey="Dediego M" first="Marta L" last="Dediego">Marta L. Dediego</name></author><author><name sortKey="Enjuanes, Luis" sort="Enjuanes, Luis" uniqKey="Enjuanes L" first="Luis" last="Enjuanes">Luis Enjuanes</name></author><author><name sortKey="Guo, Deyin" sort="Guo, Deyin" uniqKey="Guo D" first="Deyin" last="Guo">Deyin Guo</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2008">2008</date><idno type="RBID">pubmed:18827877</idno><idno type="pmid">18827877</idno><idno type="doi">10.1371/journal.pone.0003299</idno><idno type="wicri:Area/PubMed/Corpus">001A56</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">001A56</idno><idno type="wicri:Area/PubMed/Curation">001A56</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">001A56</idno><idno type="wicri:Area/PubMed/Checkpoint">001B26</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">001B26</idno><idno type="wicri:Area/Ncbi/Merge">001D61</idno><idno type="wicri:Area/Ncbi/Curation">001D61</idno><idno type="wicri:Area/Ncbi/Checkpoint">001D61</idno><idno type="wicri:Area/Main/Merge">003193</idno><idno type="wicri:Area/Main/Curation">003106</idno><idno type="wicri:Area/Main/Exploration">003106</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Genome-wide analysis of protein-protein interactions and involvement of viral proteins in SARS-CoV replication.</title><author><name sortKey="Pan, Ji An" sort="Pan, Ji An" uniqKey="Pan J" first="Ji'An" last="Pan">Ji'An Pan</name><affiliation wicri:level="4"><nlm:affiliation>State Key Laboratory of Virology and Modern Virology Research Centre, College of Life Sciences, Wuhan University, Wuhan, People's Republic of China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>State Key Laboratory of Virology and Modern Virology Research Centre, College of Life Sciences, Wuhan University, Wuhan</wicri:regionArea><placeName><settlement type="city">Wuhan</settlement><region type="région">Hubei</region></placeName><orgName type="university">Université de Wuhan</orgName></affiliation></author><author><name sortKey="Peng, Xiaoxue" sort="Peng, Xiaoxue" uniqKey="Peng X" first="Xiaoxue" last="Peng">Xiaoxue Peng</name></author><author><name sortKey="Gao, Yajing" sort="Gao, Yajing" uniqKey="Gao Y" first="Yajing" last="Gao">Yajing Gao</name></author><author><name sortKey="Li, Zhilin" sort="Li, Zhilin" uniqKey="Li Z" first="Zhilin" last="Li">Zhilin Li</name></author><author><name sortKey="Lu, Xiaolu" sort="Lu, Xiaolu" uniqKey="Lu X" first="Xiaolu" last="Lu">Xiaolu Lu</name></author><author><name sortKey="Chen, Yingzhao" sort="Chen, Yingzhao" uniqKey="Chen Y" first="Yingzhao" last="Chen">Yingzhao Chen</name></author><author><name sortKey="Ishaq, Musarat" sort="Ishaq, Musarat" uniqKey="Ishaq M" first="Musarat" last="Ishaq">Musarat Ishaq</name></author><author><name sortKey="Liu, Dan" sort="Liu, Dan" uniqKey="Liu D" first="Dan" last="Liu">Dan Liu</name></author><author><name sortKey="Dediego, Marta L" sort="Dediego, Marta L" uniqKey="Dediego M" first="Marta L" last="Dediego">Marta L. Dediego</name></author><author><name sortKey="Enjuanes, Luis" sort="Enjuanes, Luis" uniqKey="Enjuanes L" first="Luis" last="Enjuanes">Luis Enjuanes</name></author><author><name sortKey="Guo, Deyin" sort="Guo, Deyin" uniqKey="Guo D" first="Deyin" last="Guo">Deyin Guo</name></author></analytic><series><title level="j">PloS one</title><idno type="eISSN">1932-6203</idno><imprint><date when="2008" type="published">2008</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Genes, Reporter</term><term>Genome, Viral</term><term>Protein Binding</term><term>SARS Virus (physiology)</term><term>Transcription, Genetic</term><term>Two-Hybrid System Techniques</term><term>Viral Proteins (genetics)</term><term>Viral Proteins (metabolism)</term><term>Viral Proteins (physiology)</term><term>Virus Replication (physiology)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Gènes rapporteurs</term><term>Génome viral</term><term>Liaison aux protéines</term><term>Protéines virales (génétique)</term><term>Protéines virales (métabolisme)</term><term>Protéines virales (physiologie)</term><term>Réplication virale (physiologie)</term><term>Techniques de double hybride</term><term>Transcription génétique</term><term>Virus du SRAS (physiologie)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Viral Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Viral Proteins</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Protéines virales</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Protéines virales</term></keywords><keywords scheme="MESH" qualifier="physiologie" xml:lang="fr"><term>Protéines virales</term><term>Réplication virale</term><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>SARS Virus</term><term>Viral Proteins</term><term>Virus Replication</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Genes, Reporter</term><term>Genome, Viral</term><term>Protein Binding</term><term>Transcription, Genetic</term><term>Two-Hybrid System Techniques</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Gènes rapporteurs</term><term>Génome viral</term><term>Liaison aux protéines</term><term>Techniques de double hybride</term><term>Transcription génétique</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Analyses of viral protein-protein interactions are an important step to understand viral protein functions and their underlying molecular mechanisms. In this study, we adopted a mammalian two-hybrid system to screen the genome-wide intraviral protein-protein interactions of SARS coronavirus (SARS-CoV) and therefrom revealed a number of novel interactions which could be partly confirmed by in vitro biochemical assays. Three pairs of the interactions identified were detected in both directions: non-structural protein (nsp) 10 and nsp14, nsp10 and nsp16, and nsp7 and nsp8. The interactions between the multifunctional nsp10 and nsp14 or nsp16, which are the unique proteins found in the members of Nidovirales with large RNA genomes including coronaviruses and toroviruses, may have important implication for the mechanisms of replication/transcription complex assembly and functions of these viruses. Using a SARS-CoV replicon expressing a luciferase reporter under the control of a transcription regulating sequence, it has been shown that several viral proteins (N, X and SUD domains of nsp3, and nsp12) provided in trans stimulated the replicon reporter activity, indicating that these proteins may regulate coronavirus replication and transcription. Collectively, our findings provide a basis and platform for further characterization of the functions and mechanisms of coronavirus proteins.</div></front></TEI><affiliations><list><country><li>République populaire de Chine</li></country><region><li>Hubei</li></region><settlement><li>Wuhan</li></settlement><orgName><li>Université de Wuhan</li></orgName></list><tree><noCountry><name sortKey="Chen, Yingzhao" sort="Chen, Yingzhao" uniqKey="Chen Y" first="Yingzhao" last="Chen">Yingzhao Chen</name><name sortKey="Dediego, Marta L" sort="Dediego, Marta L" uniqKey="Dediego M" first="Marta L" last="Dediego">Marta L. Dediego</name><name sortKey="Enjuanes, Luis" sort="Enjuanes, Luis" uniqKey="Enjuanes L" first="Luis" last="Enjuanes">Luis Enjuanes</name><name sortKey="Gao, Yajing" sort="Gao, Yajing" uniqKey="Gao Y" first="Yajing" last="Gao">Yajing Gao</name><name sortKey="Guo, Deyin" sort="Guo, Deyin" uniqKey="Guo D" first="Deyin" last="Guo">Deyin Guo</name><name sortKey="Ishaq, Musarat" sort="Ishaq, Musarat" uniqKey="Ishaq M" first="Musarat" last="Ishaq">Musarat Ishaq</name><name sortKey="Li, Zhilin" sort="Li, Zhilin" uniqKey="Li Z" first="Zhilin" last="Li">Zhilin Li</name><name sortKey="Liu, Dan" sort="Liu, Dan" uniqKey="Liu D" first="Dan" last="Liu">Dan Liu</name><name sortKey="Lu, Xiaolu" sort="Lu, Xiaolu" uniqKey="Lu X" first="Xiaolu" last="Lu">Xiaolu Lu</name><name sortKey="Peng, Xiaoxue" sort="Peng, Xiaoxue" uniqKey="Peng X" first="Xiaoxue" last="Peng">Xiaoxue Peng</name></noCountry><country name="République populaire de Chine"><region name="Hubei"><name sortKey="Pan, Ji An" sort="Pan, Ji An" uniqKey="Pan J" first="Ji'An" last="Pan">Ji'An Pan</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/SrasV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 003106 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 003106 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Sante
|area= SrasV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= pubmed:18827877
|texte= Genome-wide analysis of protein-protein interactions and involvement of viral proteins in SARS-CoV replication.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:18827877" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \
| NlmPubMed2Wicri -a SrasV1
| This area was generated with Dilib version V0.6.33. |  |